《植物生理学报》 2015, 51(8): 1347-1349

改良NH₄F掩蔽法在测定植物组织二价铁含量中的应用

倪琳琳¹, 侯炤琪¹, 封士达¹, 吴晨阳³, 陆小龙², 韦军^1,*

扬州大学¹园艺与植物保护学院, ²测试中心, 江苏扬州225009; ³宿迁学院, 江苏宿迁223800

通信作者：韦军；E-mail: weijun@yzu.edu.cn；Tel: 13004326981

摘　要：

针对NH₄F掩蔽法测定二价铁含量中存在的问题, 将此测定方法改良为: 对提取液不用浓硫酸或盐酸调节pH, 直接先加入0.15%邻菲罗啉溶液2 mL, 再加入3 mol•L^-1 NH₄F溶液2 mL, 决定系数和变异系数分别为0.9998和10.23%, 回归率达到了100.82%, 适合植物组织中二价铁含量的精确测定。

关键词：植物组织; Fe²⁺; NH₄F掩蔽法

收稿：2015-04-30   修定：2015-07-14

资助：江苏省高校专业学位研究生科研实践计划项目(SJLX_0614)。

Application of Improved NH₄F Masking Method in Determination of Ferrous Iron in Plant Tissues

NI Lin-Lin¹, HOU Zhao-Qi¹, FENG Shi-Da¹, WU Chen-Yang³, LU Xiao-Long², WEI Jun^1,*

¹College of Horticulture and Plant Protection, ²Testing Center, Yangzhou University, Yangzhou, Jiangsu 225009, China; ³Suqian College, Suqian, Jiangsu 223800, China

Corresponding author: WEI Jun; E-mail: weijun@yzu.edu.cn; Tel: 13004326981

Abstract:

In order to solve the problems of ferrous iron contents in plant tissues, NH₄F masking method were improved in this paper. The results showed that the determination coefficient and coefficient of variation were 0.9998 and 10.23% respectively and the recovery was up to 100.82%, when 0.15% phenanthroline 2 mL and 3 mol•L^-1 NH₄F 2 mL were added in turn without sulfuric acid or hydrochloric acid to adjust pH.

Key words: plant tissue; ferrous iron; NH₄F masking method